Liquid chromatographic-fluorometric analysis of paralytic shellfish toxins

摘要: Alkaline oxidation of paralytic shellfish toxins with ten-butyl hydroperoxide yielded highly fluorescent derivatives. Even gonyautoxins I and IV neosaxitoxin, which were nonfluorescing by previously proposed hydrogen peroxide oxidations, successfully converted to compounds. A continuous toxin analyzer was constructed incorporating the reaction into a high pressure liquid chromatographic system. The limit detection within range 0.04 ~ 2.2 nmol for V, neosaxitoxin saxitoxin.