Beclin1 overexpression suppresses tumor cell proliferation and survival via an autophagy‑dependent pathway in human synovial sarcoma cells.

摘要: Beclin1 is an important autophagy‑related prot-ein, which involved in both autophagy and apoptosis. In recent years, the antitumor effect of has received increased attention. present study, we established a stable Beclin1‑overexpressing cell line with SW982 human synovial sarcoma cells. We found that overexpression decreased viability, inhibited proliferation induced apoptosis The expression levels Bcl‑2 PCNA were decreased, while cleaved‑caspase‑3 cleaved‑PARP increased. closely related autophagy, thus markers LC3 p62 detected by western blot analysis, transmission electron microscopy was used to observe autophagosomes. results showed level LC3II decreased. Moreover, many double membrane‑enclosed autophagosomes cells overexpression, indicated autophagic activity enhanced. To explore on viability cells, Atg5 knocked down using siRNA inhibit activity. contributed decrease viability. Knockdown apoptotic rate overexpression. increased, also Akt/Bcl‑2/caspase‑9 pathway involved. phosphorylation AKT positively correlated cleavage caspase‑9 inhibition autophagy. Altogether, our suggested