Intrathecal clonidine decreases spinal nitric oxide release in a rat model of complete Freund's adjuvant induced inflammatory pain.
作者： C.-J. Wang , L.-C. Yang , C.-R. Lin , Y.-C. Chuang , J.-T. Cheng
关键词: Clonidine 、 Cerebrospinal fluid 、 Hyperalgesia 、 Spinal cord 、 Anesthesia 、 Chronic pain 、 Medicine 、 Nitric oxide 、 Agonist 、 Pharmacology 、 Nociception
摘要: A long-lasting antihyperalgesic effect has been demonstrated for intrathecal (IT) clonidine, an alpha2-adrenergic agonist. In the present study, mechanism and effects of IT clonidine were examined post-treatment in a rat model Complete Freund's Adjuvant (CFA)-induced inflammatory hyperalgesia. Using chronic spinal cord dialysis, we adjuvant-induced inflammation on release nitric oxide (NO) development pain assessed antinociceptive mechanisms agonist, (IT). Chronic, persistent was induced by left hind paw injection 0.3 ml CFA prepared mixture with Mycobacterium butyricum. Rats randomly assigned to groups receiving discrete doses 1, 10 or 50 μg, 3 24 hr post-inflammation. Measurement total NO x (NO + $${\text{NO}}_{2 - } {\text{NO}}_{3 $$ ) used determine NO into cerebrospinal fluid. Rat thermal antinociception using radiant heat hyperalgesia model. resulted significant throughout four days observation. dose-dependent suppression observed after treatment. Evidence from this CFA-induced suggests that clonidine's act through inhibition release.
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