Temporal changes in matrix metalloproteinases, their inhibitors, and cathepsins in mouse pubic symphysis during pregnancy and postpartum.

摘要: Remodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The involvement endogenous proteins such as matrix metalloproteinases (MMPs), tissue inhibitors (TIMPs), cathepsins these phenomena remains unclear. In this work, we used a combination immunolocalization, protein expression/activity, relative messenger RNA (mRNA) expression to examine changes selected MMPs (-2, -9, -8), TIMPs (-1 -2), (B K) during pregnancy postpartum mice. Immunohistochemistry revealed presence all cytoplasm chondrocytes, fibrochondrocytes, fibroblast-like cells interpubic tissues. Zymography showed increases active forms MMP-2 -9 primarily on days 15 19 pregnancy. Western blotting enhanced MMP-8 12 pregnancy, with no B K. Matrix 2, TIMP-1 -2, cathepsin had significant gene throughout These findings indicate that there variations activity may have an important role remodeling events.