Effects of prunetin on the proteolytic activity, secretion and gene expression of MMP-3 in vitro and production of MMP-3 in vivo.
作者： Dae Cheol Nam , Bo Kun Kim , Hyun Jae Lee , Hyun-Dae Shin , Choong Jae Lee
DOI: 10.4196/KJPP.2016.20.2.221
关键词: Immunology 、 Western blot 、 Matrix metalloproteinase 、 Secretion 、 In vivo 、 Prunetin 、 Cell biology 、 Chemistry 、 In vitro 、 Chondrocyte 、 Gene expression
摘要: We investigated whether prunetin affects the proteolytic activity, secretion, and gene expression of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well vivo production MMP-3 rat knee joint to evaluate potential chondroprotective eff ect prunetin. Rabbit chondrocytes were a monolayer, reverse transcription-polymerase chain reaction (RT-PCR) was used measure interleukin-1β (IL-1β)-induced MMP-3, MMP-1, MMP-13, disintegrin metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5. In effects on IL-1β-induced secretion activity using western blot analysis casein zymography, respectively. The protein also examined vivo. results follows: (1) inhibited ADAMTS-4, ADAMTS-5; (2) MMP-3; (3) suppressed These suggest that can regulate expression, by directly acting chondrocytes.
sci-hub.se 参考文章(31)
Takako Hattori, Shunji Kojima, Takuo Kuboki, Manabu Kanyama, Masaharu Takigawa, Atsushi Yamashita, Takuo Fujisawa, Matrix metalloproteinases and tissue inhibitors of metalloproteinases in synovial fluids of patients with temporomandibular joint osteoarthritis. Journal of Orofacial Pain. ,vol. 14, pp. 20- 30 ,(2000)
H J Mankin, The response of articular cartilage to mechanical injury. The Journal of Bone & Joint Surgery. ,vol. 64, pp. 460- 466 ,(1982) , 10.2106/00004623-198264030-00022
Hyun Jae Lee, Su Yel Lee, Mi Nam Lee, Jang-Hyun Kim, Gyu Tae Chang, Jeong Ho Seok, Choong Jae Lee, Inhibition of Secretion, Production and Gene Expression of Mucin from Cultured Airway Epithelial Cells by Prunetin Phytotherapy Research. ,vol. 25, pp. 1196- 1200 ,(2011) , 10.1002/PTR.3362
Peter, S. Burrage, Matrix Metalloproteinases: Role In Arthritis Frontiers in Bioscience. ,vol. 11, pp. 529- 543 ,(2006) , 10.2741/1817
H. Birkedal-Hansen, W.G.I. Moore, M.K. Bodden, L.J. Windsor, B. Birkedal-Hansen, A. DeCarlo, J.A. Engler, Matrix Metalloproteinases: A Review Critical Reviews in Oral Biology & Medicine. ,vol. 4, pp. 197- 250 ,(1993) , 10.1177/10454411930040020401
Tae-Gue Ahn, Gabsik Yang, Heon-Myung Lee, Myung-Dong Kim, Ho-Young Choi, Kyoung-Sik Park, Sun-Dong Lee, Yoon-Bum Kook, Hyo-Jin An, Molecular mechanisms underlying the anti-obesity potential of prunetin, an O-methylated isoflavone Biochemical Pharmacology. ,vol. 85, pp. 1525- 1533 ,(2013) , 10.1016/J.BCP.2013.02.020
Lisa A. Neuhold, Loran Killar, Weiguang Zhao, Mei-Li A. Sung, Linda Warner, John Kulik, James Turner, William Wu, C. Billinghurst, T. Meijers, A. Robin Poole, Philip Babij, Louis J. DeGennaro, Postnatal expression in hyaline cartilage of constitutively active human collagenase-3 (MMP-13) induces osteoarthritis in mice Journal of Clinical Investigation. ,vol. 107, pp. 35- 44 ,(2001) , 10.1172/JCI10564
Patrick Garnero, Jean-Charles Rousseau, Pierre D. Delmas, Molecular basis and clinical use of biochemical markers of bone, cartilage, and synovium in joint diseases. Arthritis & Rheumatism. ,vol. 43, pp. 953- 968 ,(2000) , 10.1002/1529-0131(200005)43:5<953::AID-ANR1>3.0.CO;2-Q
T. Aigner, L. McKenna, Molecular pathology and pathobiology of osteoarthritic cartilage. Cellular and Molecular Life Sciences. ,vol. 59, pp. 5- 18 ,(2002) , 10.1007/S00018-002-8400-3
Frank Echtermeyer, Jessica Bertrand, Rita Dreier, Ingmar Meinecke, Katja Neugebauer, Martin Fuerst, Yun Jong Lee, Yeong Wook Song, Christine Herzog, Gregor Theilmeier, Thomas Pap, Syndecan-4 regulates ADAMTS-5 activation and cartilage breakdown in osteoarthritis Nature Medicine. ,vol. 15, pp. 1072- 1076 ,(2009) , 10.1038/NM.1998