Cold storage of Acartia tonsa eggs: a practical use in ecotoxicological studies.

摘要: The calanoid copepod Acartia tonsa has been recommended as a marine organism for ecotoxicological tests due to its wide distribution, short life cycle and high productivity. This species is used in acute chronic toxicity assess water sediment quality; egg hatching success the survival of first larval stages are considered endpoints. Toxicity test protocols require large number organisms an appropriate culture system. Eggs stored under conditions that delay could ensure sufficient quantities biological materials tests. In current study early-spawned eggs were at 3 °C (±1) up 240 days their was evaluated on monthly basis. Our results showed percentage 30 days >80 % decreased by about 8 % every 20 days storage, 120 days. A further increase time cold storage brought significant reduction, statistical term, compared with control group (43.69 ± 22.19 %). Almost 50 % hatched or died during period, more than 80 % lost after periods longer 150 days. To verify suitability test, 48 h performed using nickel chloride referent toxicant. 30, 60, 90 120 days gave EC50 values ranging from 0.130 0.221 mg L(-1), similar value recorded eggs, suggesting these can be open new possibilities wider use Mediterranean strain A.