Independent Control of Fiber Development and Nitrate Reduction in Cultured Cotton Ovules

摘要: Several lines of evidence implicate ammonium as an important factor in the growth and development cotton ( Gossypium hirsutum L.) ovules cultured vitro. For example, at 28 C require indoleacetic acid (IAA) either or gibberellic (GA 3 ) medium for fiber development, whereas 34 only IAA. Because this effect supply, it seemed possible that hormones increased temperature were also promoting availability reduced nitrogen by induction nitrate reductase activity ovules. This possibility was tested. In vivo, where received mostly very little nitrate, they did not display appreciable even when forced into ovary wall transpiration. After initiation culture, became freely available to their rapidly. Treatment with ammonium, GA , IAA, had no upon induction. It is concluded hormone, effects on are independent a general substrate growth.