An alternative HER-2/neu transcript of 8 kb has an extended 3'UTR and displays increased stability in SKOV-3 ovarian carcinoma cells.

摘要: Abstract HER-2/ neu is a potent oncogene that predicts poor outcome when overexpressed in ovarian cancer. The SKOV-3 carcinoma cell line, one of the only models for HER2-driven cancer, expresses major uncharacterized 8-kb alternative HER-2 transcript. Objectives. aim this study was to characterize structure and determine origin sequence examine possible role transcript overexpression gene. Methods. investigated using polymerase chain reaction (PCR) nucleotide sequencing cDNA clones. PCR analysis genomic DNA used assess stability mRNA assessed by Northern blot RNA extracted from cells treated with transcriptional inhibitors. Results. Similar 5′UTR coding but an extended 3′UTR were contained compared well-characterized 4.5-kb Genomic had continuity between novel adjacent terminal exon sequence. half-life 13 h 5.5 ( P Conclusions. generated polyadenylation site usage rather than gene rearrangement. Since contains found at 3′ end normal gene, it could be expressed other cells. Increased may confer selective advantage providing enhanced expression.