RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK.
作者： Amotz Ziv-Av , Nissim David Giladi , Hae Kyung Lee , Simona Cazacu , Susan Finniss
关键词: Cell biology 、 RNA interference 、 Biology 、 Glioma 、 Plasma protein binding 、 Cell migration 、 Actin 、 Signal transduction 、 Transfection 、 Immunoprecipitation
摘要: Glioblastoma (GBM) are characterized by increased invasion into the surrounding normal brain tissue. RTVP-1 is highly expressed in GBM and regulates migration of glioma cells. To further study effects we performed a pull-down assay using His-tagged followed mass spectrometry found that was associated with actin polymerization regulator, N-WASP. This association validated co-immunoprecipitation FRET analysis. We cell spreading, these were at least partly mediated Another protein which to interact hnRNPK. has been recently reported associate inhibit effect N-WASP on spreading. hnRNPK decreased migration, spreading Using interactions In addition, overexpression summary, report via interaction interfering inhibitory function this protein.
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