Immunohistochemical demonstration of human cardiac innervation before and after transplantation.

摘要: Knowledge about the distribution and origins of peptide-containing nerves in innervated transplanted heart is lacking. Immunohistochemical histochemical techniques were used to visualize human cardiac innervation before after transplantation. In recipient nerve fibers fascicles displayed immunoreactivity for general neural (protein gene product 9.5 synaptophysin) Schwann cell markers (S-100). A major proportion neuropeptide tyrosine hydroxylase immunofluorescence staining. Subpopulations contained somatostatin, vasoactive intestinal polypeptide, calcitonin gene-related peptide, substance P- or neurokinin-like immunoreactivity, acetylcholinesterase activity. Tissues from allografts (5 weeks 63 months transplantation) ganglion cells that positive immunoreactive markers. These less numerous than hearts rarely immunostaining. Atrial natriuretic peptide was localized myocardial as well explanted postmortem hearts. While most remain functionally extrinsically denervated, they appear contain viable intrinsic nerves, retain capacity produce atrial peptide.