Acridine orange fluorescence techniques as alternatives to traditional Giemsa staining for the diagnosis of malaria in developing countries

摘要: Traditional Giemsa-stained thick blood films were compared with 2 fluorescence microscopy techniques, acridine orange (AO) staining of thin and the quantitative buffy coat (QBC) method, for microscopical diagnosis malaria. Of 200 samples examined, 141 positive by Giemsa staining, 146 AO 137 QBC. Overall sensitivities techniques to good: 97.9% QBC 93.6%. However, parasitaemias < 100/microL sensitivity fell 41.7% whereas that was 83.3%. Both unable differentiate accurately between individual malaria species. We conclude technique alone cannot replace most purposes in an African setting. apart from species differentiation, method is appropriate laboratory developing countries.