Practical uses of acridine orange fluorescence microscopy of centrifuged blood (QBC Malaria Test) and the QBCII Hematology System in patients attending malaria clinics in Thailand.

摘要: We evaluated the detection of malaria parasites using acridine orange fluorescence microscopy centrifuged blood (AOFM/CB or "QBC Malaria Test") at two government clinics in rural Thailand. In a subgroup patients, QBC Hematology System for determination complete counts was also utilized. A Giemsa-stained thick smear (GTS) reading 100 (1,000x) microscopic fields used as standard. The AOFM/CB sensitivities were 97% overall and 95% P. falciparum (Pf). Sensitivity lower vivax (Pv) (76%). Pv sensitivity depended largely on ameboid form density. threshold to consistently detect forms estimated be 10/100 WBC (700/microliters blood). capable detecting Pf gametocytes schizonts more frequently than GTS. total rings per microliter from GTS highly correlated with number Paralens field (PMF) suggesting that could quantitatively. From technical standpoint, tropical settings Thailand this study not an obstacle use Hematology. system found useful conjunction AOFM/CB. However, patients heavily infected forms, their lymphocyte needed appropriately corrected. Overall, appears promising tool diagnosis if it is affordable developing countries.