The Discovery of the Matrix Cell, the Identification of the Multipotent Neural Stem Cell and the Development of the Central Nervous System

摘要: In the early 1960s I applied 3H-thymidine autoradiography to study of cells constituting neural tube, and found that its wall was composed solely one kind single-layered epithelial cell, which perform an elevator movement between mitotic DNA-synthetic zones in accord with cell cycle. They were identified as multipotent stem central nervous sytem (CNS) gave name matrix cells. (3)H-thymidine also revealed chronology development these cells: At first they proliferate only expand population (stage I), then switch differentiate specific neuroblasts given sequences II), finally change themselves into ependymoglioblasts, common progenitors ependymal neuroglia III). Based on findings, proposed a monophyletic view cytogenesis sytem. This theory claiming existence has long been target severe criticism not accepted among neuro-embryologists for time. Recent findings by experimental clinical neuroscientists importance have renewed interest nature biology The present paper describes how concept (multipotent vivo) emerged what come out from this over last 45 years, basic recently reconfirmed after period controversy neglect.