Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes.
作者： Alexandra Borza , Sabine Plöttner , Alexander Wolf , Claudia Behm , Silvia Selinski
DOI: 10.1007/S00204-008-0381-Z
关键词: Chemistry 、 Cytochrome P450 、 Carcinogen 、 Receptor 、 Benzo(a)pyrene 、 2-Naphthylamine 、 Polycyclic aromatic hydrocarbon 、 Biochemistry 、 Aromatic amine 、 Pyrene
摘要: Aromatic amines have been shown to cause bladder cancer. However, epithelial cells of the urinary bladder, origin cancer, may be exposed numerous substances besides aromatic amines. In present study, we analysed possible interactions between 4-aminobiphenyl (4-ABP) as well 2-naphthylamine (2-NA) and polycyclic hydrocarbon benzo[a]pyrene (B[a]P). For this purpose incubated primary porcine (PUBEC) with concentrations 1 50 microM 4-ABP without co-exposure B[a]P. As expected B[a]P increased mRNA expression cytochrome P450 1A1 (CYP1A1), whereas had no effect. when co-exposed enhanced induction CYP1A1 by This result was confirmed Western blot analysis protein expression. A similar effect for also observed cyclooxygenase-2 (COX-2) UDP-glucuronosyltransferase (UGT1). Next, studied co-exposures 2-NA Similar B[a]P-mediated CYP1A1. Our results demonstrate that some enhance influence on Ah receptor-dependent genes.
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W. N. Venables, B. D. Ripley, Modern Applied Statistics with S Springer. ,(2010) , 10.1007/978-0-387-21706-2
Paul Brennan, Olivier Bogillot, Eberhard Greiser, Jenny Chang-Claude, Jürgen Wahrendorf, Sylvaine Cordier, Karl-Heinz Jöckel, Gonzalo Lopez-Abente, Anastasia Tzonou, Paolo Vineis, Francesco Donato, Martine Hours, Consol Serra, Ulrich Bolm-Audorff, Walter Schill, Manolis Kogevinas, Paolo Boffetta, The contribution of cigarette smoking to bladder cancer in women (pooled European data). Cancer Causes & Control. ,vol. 12, pp. 411- 417 ,(2001) , 10.1023/A:1011214222810
Ji Liao, Xingzhi Xu, Michael J. Wargovich, Direct Reprobing with Anti-β-actin Antibody as an Internal Control for Western Blotting Analysis BioTechniques. ,vol. 28, pp. 216- 218 ,(2000) , 10.2144/00282BM05
Paul I. Countryman, John A. Heddle, The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes. Mutation Research. ,vol. 41, pp. 321- 331 ,(1976) , 10.1016/0027-5107(76)90105-6
Daniela Bonacker, Thomas Stoiber, Minsheng Wang, Konrad J. B�hm, Irina Prots, Eberhard Unger, Ricarda Thier, Hermann M. Bolt, Gisela H. Degen, Genotoxicity of inorganic mercury salts based on disturbed microtubule function. Archives of Toxicology. ,vol. 78, pp. 575- 583 ,(2004) , 10.1007/S00204-004-0578-8
Alexander Wolf, Alexandra Kutz, Sabine Plöttner, Claudia Behm, Herrmann M. Bolt, Wolfram Föllmann, Jürgen Kuhlmann, The effect of benzo(a)pyrene on porcine urinary bladder epithelial cells analyzed for the expression of selected genes and cellular toxicological endpoints Toxicology. ,vol. 207, pp. 255- 269 ,(2005) , 10.1016/J.TOX.2004.09.006
Fan Yang, David Bleich, Transcriptional Regulation of Cyclooxygenase-2 Gene in Pancreatic β-Cells Journal of Biological Chemistry. ,vol. 279, pp. 35403- 35411 ,(2004) , 10.1074/JBC.M404055200
Michael Fenech, The cytokinesis-block micronucleus technique: A detailed description of the method and its application to genotoxicity studies in human populations Mutation Research. ,vol. 285, pp. 35- 44 ,(1993) , 10.1016/0027-5107(93)90049-L
W. J. Conover, Mark E. Johnson, Myrle M. Johnson, A Comparative Study of Tests for Homogeneity of Variances, with Applications to the Outer Continental Shelf Bidding Data Technometrics. ,vol. 23, pp. 351- 361 ,(1981) , 10.1080/00401706.1981.10487680
D. A. Williams, A test for differences between treatment means when several dose levels are compared with a zero dose control. Biometrics. ,vol. 27, pp. 103- 117 ,(1971) , 10.2307/2528930