Characterization of OCT3/4, Nestin, NANOG, CD44 and CD24 as stem cell markers in canine prostate cancer
作者： Camila Dorotea Costa , Andre Augusto Justo , Priscila Emiko Kobayashi , Michelle M. Story , Chiara Palmieri
DOI: 10.1016/J.BIOCEL.2019.01.002
关键词: Cancer research 、 Nestin 、 CD44 、 Homeobox protein NANOG 、 Cancer cell 、 Cancer 、 Stem cell marker 、 Cancer stem cell 、 Prostate cancer 、 Biology
摘要: The cancer cell population is heterogeneous, and stem cells (CSCs) are important for tumor growth maintenance. CSC associated with different neoplastic characteristics, such as migration, resistance to apoptosis, radiation therapy chemotherapy. To increase the knowledge of CSCs in canine prostate (PC), we characterized markers PC tissues tumorspheres. We performed immunohistochemistry OCT3/4, Nestin, NANOG, CD44 CD24 10 normal prostatic tissue samples, hyperplastic (PH) samples 28 samples. Then, established two cultures profile tumorspheres grown from these cultures. Normal PH were positive only basal layer. OCT3/4 was expressed luminal tissues. There no significant difference Nestin expression among However, found higher NANOG than that Tumorspheres express CD44, indicating may be potential PC. results obtained can useful better characterize guide future studies comparative oncology.
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Nicola Mabjeesh, S Amir, Hypoxia-inducible factor (HIF) in human tumorigenesis. Histology and Histopathology. ,vol. 22, pp. 559- 572 ,(2007) , 10.14670/HH-22.559
Chiara Palmieri, Valeria Grieco, Proposal of Gleason-like grading system of canine prostate carcinoma in veterinary pathology practice Research in Veterinary Science. ,vol. 103, pp. 11- 15 ,(2015) , 10.1016/J.RVSC.2015.09.004
Mohammed Moulay, WEN Liu, Saskia Willenbrock, Katharina Anna Sterenczak, Regina Carlson, Anaclet Ngezahayo, Hugo Murua Escobar, Ingo Nolte, None, Evaluation of stem cell marker gene expression in canine prostate carcinoma- and prostate cyst-derived cell lines. Anticancer Research. ,vol. 33, pp. 5421- 5431 ,(2013)
Collene R. Jeter, Tao Yang, Junchen Wang, Hsueh-Ping Chao, Dean G. Tang, Concise Review: NANOG in Cancer Stem Cells and Tumor Development: An Update and Outstanding Questions. Stem Cells. ,vol. 33, pp. 2381- 2390 ,(2015) , 10.1002/STEM.2007
Jeff C. Liu, Tao Deng, Rajwinder S. Lehal, Jinny Kim, Eldad Zacksenhaus, Identification of tumorsphere- and tumor-initiating cells in HER2/Neu-induced mammary tumors. Cancer Research. ,vol. 67, pp. 8671- 8681 ,(2007) , 10.1158/0008-5472.CAN-07-1486
Elena Cattaneo, Ron McKay, Proliferation and differentiation of neuronal stem cells regulated by nerve growth factor Nature. ,vol. 347, pp. 762- 765 ,(1990) , 10.1038/347762A0
C. Palmieri, F.Z. Lean, S.H. Akter, S. Romussi, V. Grieco, A retrospective analysis of 111 canine prostatic samples: histopathological findings and classification. Research in Veterinary Science. ,vol. 97, pp. 568- 573 ,(2014) , 10.1016/J.RVSC.2014.11.006
Urban Lendahl, Lyle B. Zimmerman, Ronald D.G. McKay, CNS stem cells express a new class of intermediate filament protein. Cell. ,vol. 60, pp. 585- 595 ,(1990) , 10.1016/0092-8674(90)90662-X
George J. Klarmann, Elaine M. Hurt, Lesley A. Mathews, Xiaohu Zhang, Maria A. Duhagon, Tashan Mistree, Suneetha B. Thomas, William L. Farrar, Invasive prostate cancer cells are tumor initiating cells that have a stem cell-like genomic signature Clinical & Experimental Metastasis. ,vol. 26, pp. 433- 446 ,(2009) , 10.1007/S10585-009-9242-2
KATSUHITO MIYAZAWA, TAKUJI TANAKA, DAN NAKAI, NOBUYO MORITA, KOJI SUZUKI, Immunohistochemical expression of four different stem cell markers in prostate cancer: High expression of NANOG in conjunction with hypoxia-inducible factor-1α expression is involved in prostate epithelial malignancy. Oncology Letters. ,vol. 8, pp. 985- 992 ,(2014) , 10.3892/OL.2014.2274