Functional Analysis of Cotton Leaf Curl Kokhran Virus/Cotton Leaf Curl Multan Betasatellite RNA Silencing Suppressors.

摘要: In South Asia, Cotton leaf curl disease (CLCuD) is caused by a complex of phylogenetically-related begomovirus species and specific betasatellite, Multan betasatellite (CLCuMuB). The post-transcriptional gene silencing (PTGS) suppression activities the transcriptional activator protein (TrAP), C4, V2 βC1 proteins encoded Kokhran virus (CLCuKoV)/CLCuMuB were assessed in Nicotiana benthamiana. A variable degree local was observed for each viral tested, with exhibiting strongest activity only C4 preventing spread systemic silencing. CLCuKoV-encoded TrAP, CLCuMuB-encoded expressed Escherichia coli purified. TrAP shown to bind various small long nucleic acids including single-stranded (ss) double-stranded (ds) RNA DNA molecules. V2, bound ssDNA dsDNA varying affinities. Transgenic expression under constitutive 35S Cauliflower mosaic promoter dexamethasone inducible induced severe developmental abnormalities N. results indicate that homologous from even quite closely related begomoviruses may differ their suppressor mechanism action. significance these findings discussed.