Analysis of anthraquinones in cell cultures of Cinchona 'Robusta' by HPLC with photodiode array and mass spectrometry detection.
作者： Ying-Shan Han , Bertil Hofte , Robert van der Heijden , Robert Verpoorte
DOI: 10.1002/PCA.719
关键词: High-performance liquid chromatography 、 Cinchona 、 Glycoside 、 Chemistry 、 Dichloromethane 、 Chromatography 、 Acetonitrile 、 Mass spectrometry 、 Anthraquinones 、 Anthraquinone
摘要: An HPLC-PAD-ESI/MS method has been developed for the analysis of anthraquinones in cell cultures Cinchona ‘Robusta’. Using a C18 column and gradient elution with mobile phase system containing acetonitrile, water trifluoroacetic acid, satisfactory separation both anthraquinone glycosides aglycones crude dichloromethane extract could be obtained. Robustaquinone B was identified as major extracts, another five were tentatively from spectroscopic data. A number minor unknown compounds detected distinguished known anthraquinones. isocratic quantitative determination robustaquinone also developed. Copyright © 2003 John Wiley & Sons, Ltd.
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