Axotomy-induced cytoskeleton changes in unmyelinated mammalian central nervous system axons

摘要: Oligodendrocyte-derived myelin retards the ability of CNS axons to regenerate following transection. The intrinsic response an axotomy insult may be vastly different in absence myelin. However, paucity adequate experimental models has limited detailed investigation cellular behaviour axon transection unmyelinated environment. In this study we perform laser-induced porcine retinal ganglion cell axon, a physiologically unmyelinated, mature that is structurally similar humans infer knowledge about axonal Axotomy-induced changes neuronal cytoskeleton and supporting astrocytes during early stages after are delineated by examining sequence neurofilament subunit, microtubule (TUB), associated protein (MAP), glial fibrillary acidic (GFAP) terminal deoxynucleotidyl transferase biotin-dUTP nick end labelling (TUNEL) modification. Axonal induced increase expression light at regions within, immediately adjacent to, sites axotomy. Other subunits were not altered Unlike myelinated where GFAP staining within hypertrophic scars have been shown inhibit repair demonstrate decrease increased or preserved expression. TUB MAP proteins what previously described axons. This provides fundamental insights into astrocyte acutely demonstrates series degenerative events axons, which comparison prior reports findings report relevance understanding pathogenic mechanisms underlying neuro-degeneration CNS.