Comparison of peptide nucleic acid fluorescence in situ hybridization assays with culture-based matrix-assisted laser desorption/ionization-time of flight mass spectrometry for the identification of bacteria and yeasts from blood cultures and cerebrospinal fluid cultures

作者: A. Calderaro , M. Martinelli , F. Motta , S. Larini , M.C. Arcangeletti

DOI: 10.1111/1469-0691.12490

关键词: BacteriaFluorescence in situ hybridizationTime-of-flight mass spectrometryBiologyEnterococcus faecalisMolecular biologyBlood cultureStaphylococcus aureusPeptide nucleic acidMass spectrometry

摘要: … in situ hybridization is a molecular technology that rapidly identifies directly from liquid cultures the bacteria and yeasts … Impact of rapid in situ hybridization testing on coagulase-negative …

参考文章(30)
Clinical, Michael L. Wilson, Principles and procedures for blood cultures : approved guideline Clinical and Laboratory Standards Institute. ,(2007)
Nele Wellinghausen, Beate Wirths, Axel R Franz, Lothar Karolyi, Reinhard Marre, Udo Reischl, Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time LightCycler polymerase chain reaction (PCR) with sequence-specific probes. Diagnostic Microbiology and Infectious Disease. ,vol. 48, pp. 229- 241 ,(2004) , 10.1016/J.DIAGMICROBIO.2003.11.005
Graeme N. Forrest, Mary-Claire Roghmann, Latoya S. Toombs, Jennifer K. Johnson, Elizabeth Weekes, Durry P. Lincalis, Richard A. Venezia, Peptide Nucleic Acid Fluorescent In Situ Hybridization for Hospital-Acquired Enterococcal Bacteremia: Delivering Earlier Effective Antimicrobial Therapy Antimicrobial Agents and Chemotherapy. ,vol. 52, pp. 3558- 3563 ,(2008) , 10.1128/AAC.00283-08
Fusun Zeynep Akcam, Guler Yayli, Ersin Uskun, Onur Kaya, Canan Demir, Evaluation of the Bactec microbial detection system for culturing miscellaneous sterile body fluids. Research in Microbiology. ,vol. 157, pp. 433- 436 ,(2006) , 10.1016/J.RESMIC.2005.10.005
Sören Schubert, Kirsten Weinert, Chris Wagner, Beatrix Gunzl, Andreas Wieser, Thomas Maier, Markus Kostrzewa, Novel, improved sample preparation for rapid, direct identification from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The Journal of Molecular Diagnostics. ,vol. 13, pp. 701- 706 ,(2011) , 10.1016/J.JMOLDX.2011.07.004
Melvin P. Weinstein, Michael L. Towns, Seth M. Quartey, Stanley Mirrett, Larry G. Reimer, Giovanni Parmigiani, L. Barth Reller, The Clinical Significance of Positive Blood Cultures in the 1990s: A Prospective Comprehensive Evaluation of the Microbiology, Epidemiology, and Outcome of Bacteremia and Fungemia in Adults Clinical Infectious Diseases. ,vol. 24, pp. 584- 602 ,(1997) , 10.1093/CLIND/24.4.584
P. R. S. Lagace-Wiens, H. J. Adam, J. A. Karlowsky, K. A. Nichol, P. F. Pang, J. Guenther, A. A. Webb, C. Miller, M. J. Alfa, Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time Journal of Clinical Microbiology. ,vol. 50, pp. 3324- 3328 ,(2012) , 10.1128/JCM.01479-12
D. Martiny, L. Busson, I. Wybo, R. A. El Haj, A. Dediste, O. Vandenberg, Comparison of the Microflex LT and Vitek MS Systems for Routine Identification of Bacteria by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Journal of Clinical Microbiology. ,vol. 50, pp. 1313- 1325 ,(2012) , 10.1128/JCM.05971-11
A. Cherkaoui, J. Hibbs, S. Emonet, M. Tangomo, M. Girard, P. Francois, J. Schrenzel, Comparison of Two Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level Journal of Clinical Microbiology. ,vol. 48, pp. 1169- 1175 ,(2010) , 10.1128/JCM.01881-09