Dictyostelium Macroautophagy Mutants Vary in the Severity of Their Developmental Defects

摘要: Macroautophagy is the major mechanism that eukaryotes use to recycle cellular components during stressful conditions. We have shown previously Atg12-Atg5 conjugation system, required for autophagosome formation in yeast, necessary Dictyostelium development. A second reaction, Aut7/Atg8 lipidation with phosphatidylethanolamine, as well a protein kinase complex and phosphatidylinositol 3-kinase are also macroautophagy yeast. In this study, we characterize mutations putative discoideum orthologues of budding yeast genes involved one each these functions, ATG1, ATG6, ATG8. All three Dictyostelium. Mutant amoebae display reduced survival nitrogen starvation degradation Mutations produce aberrant development defects varying severity. As other mutants, atg1-1, atg6-, atg8- more plaques on bacterial lawns than nitrocellulose filters. The most severe defect observed atg1-1 mutant, which does not aggregate arrests loose mounds atg6- mutants almost normal filters, producing multi-tipped aggregates mature into small fruiting bodies. distribution green fluorescent fusion marker, Atg8, both mutants.