Effect of caffeine and of pentoxifylline on the motility and metabolism of human spermatozoa.

摘要: Human spermatozoa were washed and incubated with 6 mM-caffeine or 0.15-1.2 mM-pentoxifylline. Sperm motility was measured by time-lapse photography, the rate of glycolysis release tritiated water from 1 mM-[3-3H]D-glucose mitochondrial respiration 14CO2 mM-[U-14C]-L-lactate mM-[2-14C]pyruvate. Caffeine stimulated majority to convert 'rolling' 'yawing' mode progression a concomitant increase in lateral head displacement 4.1 +/- 0.09 microns (343) 6.7 0.25 (105) (mean s.e.m. (number spermatozoa)). There 45% decline percentage progressively motile very small decrease their velocity. Pentoxifylline had only slight effect on but produced significant Both compounds increased greater than 40% elevated production smaller extent. The concentrations ATP ADP changed little. We conclude that glycolytic pathway human can respond efficiently changes energy demand.