Interplay of lncRNA H19/miR-675 and lncRNA NEAT1/miR-204 in breast cancer.

摘要: Long noncoding RNAs (lncRNAs) are frequently precursor of microRNAs (miRNAs) or act as competing endogenous (ceRNAs) to interact with miRNAs. To better understand the shared impact lncRNAs and miRNAs in regulatory post-transcriptional network, we focused here on relationships between (a) lncRNA H19 miR-675, (b) NEAT1 miR-204, (c) HOTAIR miR-331 plasma early breast cancer (BC) patients. We quantified each RNA samples 63 BC patients 10 healthy women by quantitative real-time PCR. In cell culture experiments, influence these (ncRNAs) proliferation apoptosis line MCF-7 was examined. Plasma levels (P = 0.030), (P = 0.012) were deregulated compared women. both cohorts, concentrations correlated those miR-675 (P = 0.0001). Higher (P = 0.001) along lower (P = 0.007) higher miR-204 (P = 0.017) (P = 0.030) detected HER2-positive other subgroups. Whereas expression below detection level, nodal status (P = 0.002) recurrence (P = 0.012). a competitive ncRNAs also documented. Our findings describe relationship H19/miR-675 NEAT1/miR-204 different subtypes; addition, they reveal an interplay network cells, which should be considered search for new diagnostic therapeutic markers.