H19mRNA-like Noncoding RNA Promotes Breast Cancer Cell Proliferation through Positive Control by E2F1

摘要: The imprinted H19 gene has riboregulatory functions. We show here that transcription is up-regulated during the S-phase of growth-stimulated cells and promoter activated by E2F1 in breast cancer cells. repression pRb E2F6 confirms E2F1-dependent control promoter. Consistently, we demonstrate chromatin immunoprecipitation assays endogenous recruited to vivo. functionality E2F sites was further confirmed gel shift mutagenesis experiments, revealing these are required for binding response exogenous expression serum stimulation. Furthermore, overexpression confers a growth advantage on released from arrest as well asynchronously growing knockdown small interfering RNA duplexes impedes entry both wild-type stably H19-transfected Based findings, conclude actively linked promote cell cycle progression This clearly supports oncogenic function tumor genesis.