Suppressed tumour growth and enhanced chemosensitivity by RNA interference targeting Aurora-A in the PC3 human prostate cancer model.
作者： Masafumi Kumano , Hideaki Miyake , Tomoaki Terakawa , Junya Furukawa , Masato Fujisawa
DOI: 10.1111/J.1464-410X.2009.09047.X
关键词: Pathology 、 Small hairpin RNA 、 Expression vector 、 Cancer 、 Cancer research 、 Growth inhibition 、 Prostate cancer 、 Medicine 、 Cytotoxic T cell 、 Immunohistochemistry 、 Docetaxel
摘要: OBJECTIVES To investigate the inhibitory effects of Aurora-A expression in prostate cancer cells on their growth and chemosensitivity. PATIENTS AND METHODS Aurora-A radical prostatectomy specimens obtained from 193 patients were evaluated by immunohistochemical staining. We then established PC3 which vector containing short-hairpin RNA (shRNA) targeting was introduced (PC3/sh-A). The sensitivity to docetaxel PC3/sh-A compared with those transfected control alone (PC3/C). RESULTS Immunohistochemistry showed that there various levels most tissues, significantly related Gleason score. Expression both mRNA protein ≈20% PC3/C. In vitro worse than PC3/C, proportion G2-M phase greater 50% concentration decreased 67% Tumour volume nude mice injected smaller Furthermore, treatment bearing tumour (10 mg/kg, once weekly for 4 weeks) achieved a synergistic cytotoxic effect, despite lack an enhanced antitumour effect PC3/C tumours. CONCLUSIONS The suppression using shRNA could be useful therapeutic strategy against androgen-independent cancer, through inhibition as well chemosensitivity.
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