CD44 loss in gastric stromal tumors as a prognostic marker.

摘要: CD44 loss in gastric stromal tumors as a prognostic marker Background: The adhesion molecule (CD44s; CD44H) and its isoforms (CD44v3-6 v9) are preferentially expressed by different cell types. These transmembrane glycoproteins involved cell-cell cell-matrix interactions trafficking and, thus, may play role tumor metastasis and/or local invasion. expression pattern of CD44s variant isoforms, particularly CD44v6 CD44v9, some neoplasms, including soft tissue tumors, correlates with clinical course outcome. behavior gastrointestinal (GIST) is site specific; however, other reliable predictors outcome have not been identified. Thus, the value isoform GIST were evaluated immunohistochemistry microarrays. Design: Paraffin- embedded formalin-fixed cores (129: 103 26 normal stomach smooth muscle) from 33 patients data collected used for construction One to five each patient specimen (mean = 3 cores/patient). Array slides stained anti-CD44s (CD44H) antibodies v3, v4, v5, v6, v9 isomers. staining intensity scored semi quantitatively without knowledge identity or outcome: 0 no; 1 weak; 2 moderate; moderate strong; 4 strong. scores multiple same averaged; nonneoplastic muscle was similarly graded. compared Results: GIST, 0.8 30 cm size, followed 111 months median follow-up 7 17.5 months). overall survival 25 months. Nine (27%) had metastases, 9 recurrent disease, died disease (9-111 months; mean 39 23 All 18 >2+ alive at last (1-62 3.5 11 More than half (53%) less equal to2+ 38 months); surviving to2 5 (2-22 6.5 log rank P 0.07). majority variably positive CD44v3 CD44v4, but there minimal (number cases level) directed against Conclusion: preliminary results suggest that although GISTs express variants, only positivity correlating poor