Adeno-Associated Virus Serotype 4 (AAV4) and AAV5 Both Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic Acid Linkage Specificity

摘要: Adeno-associated virus serotype 4 (AAV4) and AAV5 have different tropisms compared to AAV2 each other. We recently reported that α2-3 sialic acid is required for binding transduction. In this study, we characterized AAV4 transduction found it also binds acid, but the specificity significantly from AAV5. can hemagglutinate red blood cells several species, whereas hemagglutinates only rhesus monkey cells. Treatment of with trypsin inhibited hemagglutination both AAV5, suggesting agglutinin a protein. Cos neuraminidases indicated bound acid. However, resialylation experiments neuraminidase-treated demonstrated α2–3 O-linked N-linked Similarly, acid-deficient CHO supported same conclusion. The difference in linkage was confirmed by incubated either or inhibitors glycosylation. Furthermore, blocked soluble could be α2-6 These results suggest require acid-containing glycoproteins target they further explain tropism