Osteosarcoma tissue-engineered model challenges oxidative stress therapy revealing promoted cancer stem cell properties.

摘要: The use of oxidative stress generated by Cold Atmospheric Plasma (CAP) in oncology is being recently studied as a novel potential anti-cancer therapy. However, the beneficial effects CAP for treating osteosarcoma have mostly been demonstrated 2-dimensional cultures cells, which do not mimic complexity 3-dimensional (3D) bone microenvironment. In order to evaluate relevant context human disease, we developed 3D tissue-engineered model using bone-like scaffold made collagen type I and hydroxyapatite nanoparticles. Human cells cultured within showed high capacity infiltrate proliferate exhibit osteomimicry vitro. As expected, observed significantly different functional behaviors between monolayer when treated with Plasma-Activated Ringer's Solution (PAR). Our data reveal that environment only protects from PAR-induced lethality scavenging diminishing amount reactive oxygen nitrogen species CAP, but also favours stemness phenotype cells. This first study demonstrates negative effect PAR on cancer stem-like cell subpopulations biomimetic cancer. These findings will allow suitably re-focus research plasma-based therapies future.