Intermediate-level disinfection with accelerated hydrogen peroxide prevents accumulation of bacteria in Versajet™ tubing during repeated daily debridement using simulated-use testing with an inoculated pork hock.

摘要: Abstract Objective This study assesses the feasibility of using Versajet™ system (VJS) on an inoculated pork hock (PH) skin surface sequentially for 8 days with daily cleaning and intermediate-level disinfection (ILD). Methods Daily, PHs were bacteria suspended in artificial test soil (ATS). An ILD protocol accelerated hydrogen peroxide (AHP, OxivirTB®) was employed to clean disinfect VJS between debridements. Results PH contains 6.1–6.8 × 106 cfu/cm2 bacteria. Bacterial counts handpiece discharge hoses immediately after debridement PHs, before cleaning, increased throughout period (5.19–6.43 log10 cfu/mL). Cleaning reduced bacterial by 6-log. Protein, a surrogate marker organic contamination, also post-cleaning ILD. Compared maximum post-debridement level protein (57.9 μg/mL) obtained ILD, levels dropped 9.8 (handpiece) 13.8 μg/mL (discharge hose). Conclusions Disinfection hose AHP resulted 6-log reduction count 4.2 fold protein. may be feasible method serial debridements up eight days.