[6]-Dehydroshogaol, a minor component in ginger rhizome, exhibits quinone reductase inducing and anti-inflammatory activities that rival those of curcumin

摘要: Abstract Quinone reductase (QR) inducing activity, a biomarker for Phase II enzyme induction, was detected in crude ginger rhizome ethanolic extract using murine hepatoma cell (Hepa 1c1c7) bioassay. An ethyl acetate-partitioned fraction became enriched QR activity with CD value (concentration required to double specific activity) of 7 μg/ml and further separated by silica flash chromatography. The four major fractions recovered exhibited values ranging 10–20 μg/mL activity. 1 H NMR analysis revealed an abundance gingerols the first 3 fractions, this led comparative assessment anti-inflammatory activities among [6]-gingerol structurally related vanilloids from other spices. [6]-Dehydroshogaol (DHSG), minor component fresh ginger, had (CD = 8.4 μM) comparable potency well known cancer preventive agent, curcumin (CD = 4.3 μM). In contrast, pungent showed less potent (CD = 55 μM) capsaicin weakest (CD = 103 μM). [6]-Shogaol, [6]-DHSG were also inhibitors nitric oxide (NO) synthesis, inhibiting 50% NO evolution activated macrophages at levels 10–12 μM (IC 50 values). Comparatively, IC about order magnitude greater (less potent) 90 μM 169 μM, respectively. This appears be report biological [6]-DHSG, enzyme-inducing ability shogoal gingerol.