Relevance of Ki-67 antigen expression and K-ras mutation in colorectal liver metastases.

摘要: Abstract Aims The liver is a frequent site of metastases from colorectal cancer. While these lesions are potentially amenable to surgical resection, they usually very aggressive, and recurrence frequent. Mutations the proto-oncogene K- ras thought impart strong growth signal tumour cells closely associated with development malignancies colon rectum. Hepatic cancer have notably elevated proliferative rates. present study was performed investigate relationship between proliferation or mutation prognosis following curative resection metastases. Methods Colorectal 41 patients undergoing hepatic were examined for status presence mutations. activity assessed by Ki-67 immunohistochemistry. DNA same tissue samples screened point mutations in codon 12 gene using novel microplate-based allelic-specific hybridization assay. scores then related patient survival as determined through retrospective analysis. Results Median 40 months. Patients high (≥50%) had significantly shorter median compared those low (30 vs 44 months, log-rank P =0.02). A score an independent negative prognostic factor multivariate regression analysis (relative risk=3.04, =0.036). detected 6/41 (15%), but mutational did not correlate survival. Conclusions These findings suggest that index useful predictor aggressive behaviour indicator does appear