Immune production of interferon by cultured peripheral blood mononuclear cells from calves infected with BHV1 and PI3 viruses.

摘要: Peripheral blood mononuclear cells (PBMC) from calves infected with bovine herpesvirus type 1 (BHV1) or parainfluenza 3 virus (PI3) were cultured in vitro the presence of inactivated specific antigen presented on MDBK cells. In antigen, PBMC both BHV1-infected and control produced interferon (IFN)-alpha 24 hour cultures. Altering culture conditions did not result detection immune-specific IFN by calves. However, spontaneous was detected absence cultures animals: this pH 2 labile completely neutralised antiserum to recombinant IFN-gamma. Spontaneous IFN-gamma production only seen following a second BHV1 inoculation, given four seven weeks after primary dose. contrast PI3 virus-infected produce spontaneously, but so which contained antigen. Mononuclear animals failed either IFN-alpha -gamma when virus. infection, no increase occurring subsequent inoculations. Immunospecific provides simple method for monitoring cell-mediated immunity BHV1- can be used evaluating efficacy vaccines against these viruses.