Autologous spheroid culture: a screening tool for human brain tumour invasion.

作者: Leo De Ridder , Maria Cornelissen , D De Ridder

DOI: 10.1016/S1040-8428(00)00081-0

关键词: SpheroidStereotactic biopsyMetastasisBiopsyIn vivoPathologyConfluencyBiologyBrain biopsyCell culture

摘要: Abstract Spheroids are three-dimensional cell aggregates expressing histotypic organisation in vitro comparable to tissue continuity vivo. They can be prepared from normal and tumour fragments. In the experiments presented here, dermal human spheroids brain same patient. The originates border of incision wound made effect a stereotactic biopsy. fragment collected biopsy explanted form confluent monolayers. At confluency, cells transferred into small Erlenmeyer flasks rotated at 37°C for 1–2 days rotation mediated formed. Small flaps monolayer placed on semisolid non-adhesive substrate, reorganise agar overlay spheroids. After spheroid formation, is confronted with derived spheroid. confronting pair, after adhering each other, present an invasion model vitro. functions as autologous host Putative invasive reaggregated will invade destroy it. If no morphologic changes seen spheroid; 24 tested demonstrated clear correlation between malignancy situ invasiveness So it concluded that confrontation patient has predictive value concerning malignant evolution mimics situation situ.

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