Expression of Drug Resistance-related Genes in Head and Neck Squamous Cell Carcinoma and Normal Mucosa
作者： Shitau Hirata , Osamu Katoh , Tetsuya Oguri , Hiromitsu Watanabe , Koji Yajin
DOI: 10.1111/J.1349-7006.2000.TB00863.X
关键词: Pathology 、 Topoisomerase 、 Biopsy 、 Cancer research 、 Biology 、 Drug resistance 、 Gene expression 、 Epidermoid carcinoma 、 Head and neck squamous-cell carcinoma 、 In vivo 、 Lymph node 、 Oncology
摘要: We examined the expression levels of mRNA for multidrug resistance 1 (MDR1), resistance-associated protein (MRP), human canalicular multispecific organic anion transporter (cMOAT), lung resistance-related (LRP), topoisomerase IIalpha, beta (Topo beta) and I I) genes in head neck squamous cell carcinoma (HNSCC) specimens mucosa (HNM) specimens, to elucidate their roles relation biological characteristics drug vivo. Fifty-eight samples (45 carcinomas 13 mucosa) obtained during surgical resection or biopsy from 38 patients were analyzed using quantitative reverse transcription-polymerase chain reaction (RT-PCR) method. MDR1, MRP, LRP, Topo IIbeta, gene transcripts detected all tested, but cMOAT was not them. Comparisons HNSCC with those HNM showed that IIalpha level higher than (P=0.0298). Moreover, significantly metastatic lymph node (P=0.0205). There no significant differences six genes' between exposed platinum drugs drugs. These results suggest it may be effective anticancer therapy use topoisomerase-targetting against HNSCC, especially tumors, these is associated exposure.
sci-hub.se 参考文章(47)
Zittoun R, Zhou Dc, Marie Jp, Expression of multidrug resistance-associated protein (MRP) and multidrug resistance (MDR1) genes in acute myeloid leukemia. Leukemia. ,vol. 9, pp. 1661- 1666 ,(1995)
Scheper Rj, Meijer Cj, Izquierdo Ma, van der Valk P, Broxterman Hj, Flens Mj, Giaccone G, Scheffer Gl, Broad distribution of the multidrug resistance-related vault lung resistance protein in normal human tissues and tumors. American Journal of Pathology. ,vol. 148, pp. 877- 887 ,(1996)
Yoshiyuki Kakehi, Wen‐Jeng Wu, Wun‐Jae Kim, Shinji Arao, Manabu Fukumoto, Osamu Yoshida, Comparison of multidrug resistance gene expression levels with malignant potentials and influence of chemotherapy in urothelial cancers. International Journal of Urology. ,vol. 2, pp. 309- 315 ,(1995) , 10.1111/J.1442-2042.1995.TB00005.X
MI Sandri, D Hochhauser, P Ayton, RC Camplejohn, R Whitehouse, H Turley, K Gatter, ID Hickson, AL Harris, Differential expression of the topoisomerase II alpha and beta genes in human breast cancers. British Journal of Cancer. ,vol. 73, pp. 1518- 1524 ,(1996) , 10.1038/BJC.1996.286
Giuseppe Giaccone, Franco Zunino, Giovanni Capranico, Hans Beck, Adi F. Gazdar, Multidrug sensitivity phenotype of human lung cancer cells associated with topoisomerase II expression. Cancer Research. ,vol. 52, pp. 1666- 1674 ,(1992)
L C Shea, B Gustavsson, T Horikoshi, K D Danenberg, T H Stadlbauer, M Ray, K Aigner, R Frösing, M Volkenandt, L Leichman, Quantitation of Thymidylate Synthase, Dihydrofolate Reductase, and DT-Diaphorase Gene Expression in Human Tumors Using the Polymerase Chain Reaction Cancer Research. ,vol. 52, pp. 108- 116 ,(1992)
R K Johnson, F H Drake, R D Woessner, C K Mirabelli, M R Mattern, Proliferation- and cell cycle-dependent differences in expression of the 170 kilodalton and 180 kilodalton forms of topoisomerase II in NIH-3T3 cells. Cell Growth & Differentiation. ,vol. 2, pp. 209- 214 ,(1991)
J C Wang, DNA topoisomerases: why so many? Journal of Biological Chemistry. ,vol. 266, pp. 6659- 6662 ,(1991) , 10.1016/S0021-9258(20)89545-3
Kazuhiro Kagotani, Shin-ichi Akiyama, Morimasa Wada, Michihiko Kuwano, Ken Taniguchi, Takeshi Kawabe, Kimitoshi Kohno, Mina Kawakami, Katsuzumi Okumura, Takanori Nakamura, A Human Canalicular Multispecific Organic Anion Transporter (cMOAT) Gene Is Overexpressed in Cisplatin-resistant Human Cancer Cell Lines with Decreased Drug Accumulation Cancer Research. ,vol. 56, pp. 4124- 4129 ,(1996)
Elisna Syahruddin, Tetsuya Oguri, Toshiaki Takahashi, Takeshi Isobe, Yasuhiro Fujiwara, Michio Yamakido, Differential Expression of DNA Topoisomerase IIα and IIβ Genes between Small Cell and Non‐small Cell Lung Cancer Japanese Journal of Cancer Research. ,vol. 89, pp. 855- 861 ,(1998) , 10.1111/J.1349-7006.1998.TB00640.X