P53-induced microRNA-107 inhibits proliferation of glioma cells and down-regulates the expression of CDK6 and Notch-2
作者： Lei Chen , Run Zhang , Peng Li , Yi Liu , Kun Qin
DOI: 10.1016/J.NEULET.2012.11.047
关键词: Cell biology 、 Notch 2 、 Transfection 、 Cell cycle 、 Cyclin-dependent kinase 6 、 microRNA 、 Cell growth 、 Carcinogenesis 、 Biology 、 Glioma
摘要: Abstract MicroRNAs (miRNAs) are small noncoding RNAs that function as tumor suppressors or oncogenes. MicroRNA-107 (miR-107), a transcriptional target of p53, is deregulated in many cancer cell lines. Here, we showed miR-107 down-regulated glioma tissues and lines, particular, p53-mutated U251 A172. Transfection wild-type p53 into these cells stimulated expression. To investigate the role tumorigenesis, constructed lentiviral vector overexpressing miR-107. Notably, inhibited proliferation arrested cycle at G0–G1 phase cells. Transduction Lenti-GFP-miR-107 CDK6 Notch-2 protein Our findings collectively demonstrate p53-induced suppresses brain growth down-regulates expression, supporting its suppressor utility for therapy.
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