Notch–RBP-J signaling controls the homeostasis of CD8− dendritic cells in the spleen

摘要: Signaling through Notch receptors and their transcriptional effector RBP-J is essential for lymphocyte development function, whereas its role in other immune cell types unclear. We tested the function of canonical Notch-RBP-J pathway dendritic (DC) maintenance vivo. Genetic inactivation bone marrow did not preclude DC lineage commitment but caused reduction splenic fraction. The DCs using a novel DC-specific deleter strain selective loss CD8(-) subset reduced frequency cytokine-secreting after challenge with Toll-like receptor ligands. In contrast, subsets lymph nodes tissues were unaffected. RBP-J-deficient depleted at postprogenitor stage, exhibited increased apoptosis, lost expression target gene Deltex1. spleen, found adjacent to cells expressing ligand Delta-like 1 marginal zone (MZ). Thus, signaling controls MZ, revealing an unexpected innate system.