Adipose tissue-derived stromal cells inhibit TGF-β1-induced differentiation of human dermal fibroblasts and keloid scar-derived fibroblasts in a paracrine fashion.

摘要: Background: Adipose tissue-derived stromal cells augment wound healing and skin regeneration. It is unknown whether how they can also influence dermal scarring. The authors hypothesized that adipose inhibit adverse differentiation of fibroblasts induced by the pivotal factor in scarring, namely, transforming growth (TGF)-beta. Methods: TGF-beta 1-treated adult human keloid scar-derived were incubated with cell-conditioned medium assessed for proliferation differentiation, particularly production collagen, expression SM22 alpha, development hypertrophy contractility. Results: 1-induced was abolished medium. Simultaneously, reduced SM22a gene protein TGF beta fibroblasts, their contractility also. Furthermore, strongly transcription collagen I III genes corresponding proteins. In contrast, it tipped balance matrix turnover to degradation through stimulating metalloproteinase (MMP)-1, MMP-2, MMP-14, whereas MMP-2 activity up-regulated Even end-stage myofibroblasts (i.e., fibroblasts), suppressed myofibroblast contraction expression. Conclusion: show function a paracrine fashion.