Microbicide efficacy and toxicity tests in a mouse model for vaginal transmission of Chlamydia trachomatis.

摘要: Background: Microbicides are being developed for woman-controlled protection against sexually transmitted diseases (STDs). Goal: The goal of the study was to test candidate microbicides in a mouse model preventing vaginal transmission Chlamydia trachomatis and acute toxicity columnar epithelium. Study Design: Progestin-sensitized CF-1 mice were treated vaginally with 50 μl microbicide, followed either by inoculation 10 ID C serovar D or examination epithelial surface viability stain (ethidium homodimer-1). Results: Nonoxynol-9 (N9), sodium dodecyl sulfate (SDS), chlorhexidine digluconate, BufferGel all provided significant though incomplete transmission. Other candidates, which effective vitro, no protection: κ-darrageenan, dextran sulfate, polystyrene sulfonate, Concanavalin A, wheat germ agglutinin, Phaseolus vulgaris agglutinin. surface-active agents (N9, SDS, chlorhexidine) caused toxicity: 3 days after exposure, also had friability markedly increased susceptibility Chlamydia. only tested that both protective relatively nontoxic. Conclusion: can provide highly susceptible progestin-sensitized mice. Since N9 does not inactivate Chlamydia, it likely protects killing target cells vagina. Despite ability potently kill cells, two agents, SDS chlorhexidine, failed complete protection, circumstance emphasizes importance distributing surfaces.