Fabrication of an amperometric acetylcholine esterase-choline oxidase biosensor based on MWCNTs-Fe(3)O(4)NPs-CS nanocomposite for determination of acetylcholine
作者： Ecem Özlem Bolat , Gözde Aydoğdu Tığ , Şule Pekyardımcı
DOI: 10.1016/J.JELECHEM.2016.12.041
关键词: Electrochemistry 、 Cyclic voltammetry 、 Nuclear chemistry 、 Chemistry 、 Choline oxidase 、 Detection limit 、 Dielectric spectroscopy 、 Chitosan 、 Analytical chemistry 、 Amperometry 、 Biosensor
摘要: Abstract In this paper, a novel amperometric bienzymatic biosensor based on iron (II, III) oxide nanoparticles (Fe 3 O 4 NPs), multi-walled carbon nanotubes (MWCNTs) and chitosan (CS) modified glassy electrode (GCE) was fabricated for the determination of acetylcholine (ACh). The CS used to immobilize esterase (AChE) choline oxidase (ChOx). nanocomposites Fe NPs MWCNTs were characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) cyclic voltammetry (CV). measurements detection enzymatically produced hydrogen peroxide (H 2 ). experimental parameters such as working potential, enzyme unit, pH temperature optimized. linear ranges 0.02 μmol L − 1 –0.111 μmol L 0.111 μmol L –1.87 μmol L . limit calculated 0.61 nmol L addition, showed high sensitivity, good selectivity, repeatability, reproducibility long-term stability. determine ACh levels in serum samples.
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