Deep sequencing: becoming a critical tool in clinical virology.

摘要: Population (Sanger) sequencing has been the standard method in basic and clinical DNA for almost 40 years; however, next-generation (deep) methodologies are now revolutionizing field of genomics, virology is no exception. Deep highly efficient, producing an enormous amount information at low cost a relatively short period time. High-throughput techniques have enabled significant contributions to multiples areas virology, including virus discovery metagenomics (viromes), molecular epidemiology, pathogenesis, studies how viruses escape host immune system antiviral pressures. In addition, new more affordable deep sequencing-based assays being implemented laboratories. Here, we review use current platforms focusing on three most studied viruses: human immunodeficiency (HIV), hepatitis C (HCV), influenza virus.