Identification of the major component of the estrogen-induced protein of rat uterus as the BB isozyme of creatine kinase.

摘要: We have recently shown that the estrogen-induced protein of rat uterus (IP) is indistinguishable from a constitutive in brain, and brain type gamma isozyme enolase (EC 4.2.1.11) component IP. Here we report BB creatine kinase 2.7.3.2) major The two IP components, gamma, were copurified by ammonium sulfate fractionation DEAE-cellulose chromatography. enzymes separated on reactive blue 2 agarose, which absorbs but not at 40 mMNaCl, pH 5.2. was identified as basis its specific enzyme activity, chromatographic behavior, immunoprecipitation anti-creatine antiserum. identity confirmed double isotope ratio analysis, limited protease digestion patterns, rapid increase rate synthesis response to estrogen. has been favored marker for estrogen action because early hormone both vivo vitro. identification step toward understanding function responses reveals further advantage study gene expression, possible enzymic responsive tumors.