E-ADA activity in lymphocytes of an experimental model of pythiosis treated with immunotherapy.
作者： Barbara Charlotte Bach , Daniela Bitencourt Rosa Leal , Jeandre Augusto dos Santos Jaques , Viviane do Carmo Gonçalves Souza , Jader Betsch Ruchel
DOI: 10.1002/CBF.2921
关键词: Adenosine 、 Adenine nucleotide 、 Immune system 、 Purinergic receptor 、 Immunology 、 Pythiosis 、 Pythium insidiosum 、 Immunotherapy 、 Adenosine deaminase 、 Biology
摘要: Pythiosis is a life-threatening disease caused by the oomycete Pythium insidiosum. Some authors have suggested involvement of Th2-like immune response in infected host, which leads to extensive tissue damage. The switch from Th2 Th1 pattern one hypothesis explain curative properties immunotherapy. Taking into account importance immunotherapy for pythiosis treatment and contribution adenine nucleotides immunoregulation we evaluated ecto-adenosine deaminase (E-ADA; EC 3·5.4·4) activity lymphocytes rabbits inoculated with P. insidiosum. Rabbits were 1 milliliter zoospores subcutaneously injected lateral thorax; after developing lesions, received eight doses E-ADA was measured adenosine levels quantitatively determined serum. characteristic lesions showed decreased (82·36%), triphosphate concentration (54·04%) higher (2·51 fold), when compared controls, 28 days inoculation. However, immunotherapy, an increase control (78·62%), contributing change response. Our results reinforce that participation purinergic system could be responsible Copyright © 2012 John Wiley & Sons, Ltd.
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