Optimization of bone-tissue engineering in goats

摘要: Successful bone-tissue engineering (TE) has been reported for various strategies to combine cells with a porous scaffold. In particular, the period after seeding until implantation of constructs may vary between hours and several weeks. Differences these can be reduced (a) presence extracellular matrix, (b) differentiation status cells, (c) residual potentially immunogenic serum proteins. These parameters are investigated in two types calcium phosphate scaffolds goat model ectopic bone formation. Culture-expanded bone-marrow stromal from eight goats were seeded onto hydroxyapatite granules: HA60/400 (60% porosity, 400-m average pore size) HA70/800. Scaffolds control cultured 6 days medium containing autologous or semisynthetic serum, absence dexamethasone. Other just before without serum. All conditions implanted autologously paraspinal muscles. After 12 weeks, had formed 87% all TE constructs, as demonstrated by histology. Histomorphometry indicated significantly more HA70/800 scaffolds. Furthermore, significant advantage formation was found when days. conclusion, both scaffold characteristics (porosity) strategy (culturing constructs) important TE.