Diagnostic sensitivity of immunodominant epitopes of glutamic acid decarboxylase (GAD65) autoantibodies in childhood IDDM.
作者： A. Falorni , M. Ackefors , C. Carlberg , T. Daniels , B. Persson
DOI: 10.1007/BF00400659
关键词: Immunoglobulin G 、 Radioimmunoassay 、 Endocrinology 、 Autoantibody 、 Titer 、 Recombinant DNA 、 Antibody 、 Internal medicine 、 Glutamate decarboxylase 、 Epitope 、 Biology
摘要: The prevalence and titre of epitope-specific autoantibodies to glutamic acid decarboxylase (GAD65) in 155 insulin-dependent diabetic (IDDM) 9 GAD65 antibody (Ab)-positive healthy children were determined using four GAD65/67 chimaeric molecules which discriminate among the N-terminal (N), middle (M) C-terminal (C) epitopes GAD65. Radioligand binding assays for IgGAb used immunoprecipitation vitro translated 35S-GAD. We found 116 (75%), GAD67 19 (12%) (p < 0.0001) GAD65-N-67 chimaera 25 (16%) IDDM sera. GAD67Ab almost exclusively (17 19, 89%) GAD65Ab-positive sera levels correlated with those GAD65Ab (r2 = 0.5913; p 0.009). directed GAD65-M 104 (67%), GAD65-C (67%) + C (75%) sera, indicated reactivity at least two distinct epitopes. Among nine children, (22%) also positive GAD67, (100%) C, seven (78%) GAD65-M, eight (89%) GAD65-N-67. Titres 0.007), GAD65-C-Ab 0.002) M-Ab 0.003), but not GAD65-M-Ab 0.101) significantly higher than children. conclude that are epitopes, propose antibodies specifically carboxy-terminal end may distinguish from
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