Human monoclonal islet specific autoantibodies share features of islet cell and 64 kDa antibodies.
作者： W. Richter , T. H. Eiermann , J. Endl , J. Sei�ler , S. Wolfahrt
DOI: 10.1007/BF00401152
关键词:
摘要: The first human monoclonal islet cell antibodies of the IgG class (MICA 1-6) obtained from an individual with Type 1 (insulin-dependent) diabetes mellitus were cytoplasmic selected by indirect immunofluorescence test on pancreas sections. Surprisingly, they all recognized 64 kDa autoantigen glutamate decarboxylase. In this study we investigated which typical features are represented these monoclonals. We show double testing that MICA 1-6 stain pancreatic beta cells is in agreement beta-cell specific expression contrast islet-reactive IgM antibody a pre-diabetic stained but lacked tissue specificity and must therefore be considered as polyreactive IgM-antibody. further demonstrate revealed epitope sensitivity to biochemical treatment target has been demonstrated for antibodies, used argue lipid rather than protein nature antigens. Our results provide direct evidence epitopes destroyed methanol/chloroform reveal high stability Pronase digestion compared proinsulin epitopes. Conformational decarboxylase sections such ganglioside share decarboxylase-reactive represent subgroup present antibody-positive sera.
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