Centlein, a novel microtubule-associated protein stabilizing microtubules and involved in neurite formation

摘要: We have previously reported that the centriolar protein centlein functions as a molecular link between C-Nap1 and Cep68 to maintain centrosome cohesion [1]. In this study, we identified novel microtubule-associated (MAP), directly binding purified microtubules (MTs) via its longest coiled-coil domain. Overexpression of caused profound nocodazole- cold-resistant MT bundles, which also relied on MT-binding siRNA-mediated depletion resulted in significant reduction tubulin acetylation level overall fluorescence intensity cytoplasmic acetylation. Centlein was further characterized neurons. found overexpression inhibited neurite formation retinoic acid (RA)-induced SH-SY5Y N2a cells. Taken together, propose is involved stability neuritogenesis in vivo.