The antimicrotubule drug estramustine but not irradiation induces apoptosis in malignant glioma involving AKT and caspase pathways.

摘要: Irradiation is one of the cornerstones used in treatment malignant glioma. However, effect modest and glioma cells generally display a pronounced radio-resistance. In this study, irradiation, alone combination with antimicrotubule drug estramustine (EaM), was investigated vitro using BT4C rat cell line, vivo intracerebral model used. Apoptosis detected by analysing DNA laddering, situ end labelling (ISEL) Annexin V reactivity. addition, phosphorylation status MAPK, JNK, p38, AKT, proteins involved pro- anti-apoptotic signalling pathways analysed Western blotting. did not induce apoptosis, neither nor vivo. EaM, however, induced apoptosis vitro, regardless whether EaM given alone, before or after irradiation. When were treated caspase-3 inhibitor Ac-DEVD-CHO prior to number apoptotic decreased, indicating an involvement caspase-3. The regulating are complex involve kinases such as p38 AKT. any changes expression levels these proteins. On other hand, level AKT reduced treatment, which might, part, propose how induces cells.