A new technology for mutation detection.
作者： W SHACKELFORD , S DENG , K MURAYAMA , J WANG
关键词: Chromosomal translocation 、 Genetics 、 Point mutation 、 Biology 、 Primer extension 、 COLD-PCR 、 Mutant 、 Molecular biology 、 Polymerase chain reaction 、 DNA 、 Single-nucleotide polymorphism
摘要: A sensitive, accurate, and simple method, called shifted termination assay (STA), was developed for detection of genetic mutations. The STA technology can be used to detect mutations in polymerase chain reaction (PCR)-amplified samples tissue, plasma serum that include circulating DNA containing point mutations, insertions, deletions, translocations, or single nucleotide polymorphisms (SNPs). is a multiple-base multiple-cycle primer extension based method identify mutant as little 1% mixture with 99% wild-type DNA. On the basis mutation kit, Mutector, developed. Mutector 96-well microplate format test kit colorimetric luminescent options. An initial study shows more accurate sensitive than sequencing PCR restriction enzyme analysis methods detecting from clinical samples.
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