Adenosine uptake by isolated membrane vesicles from Escherichia coli K-12
作者： Y. Komatsu
DOI: 10.1016/0005-2736(73)90226-5
关键词: Adenosine 、 Biology 、 Inosine 、 Escherichia coli 、 Caffeine 、 Showdomycin 、 Guanosine 、 Pyrimidine 、 Biochemistry 、 Guanine 、 Biophysics 、 Cell biology
摘要: Abstract 1. Three different adenosine-uptake systems were found in isolated membrane vesicles of Escherichia coli K-12 cells: the first system was strongly inhibited by adenonine nucleosides, pyrimidine nucleosides and antibiotic showdomycin but weakly guanine inosine; second adenine inosine showdomycin; third adenine. In a showdomycin-resistant mutant (Shm r -001), no longer detectable to remain active. 2. The adenosine uptake these stimulated artificial electron-donor system, ascorbate-phenazine methosulfate. reduced phenazine methosulfate-stimulated sodium azide. 3. Caffeine markedly that system. 4. Showdomycin only slightly previously loaded presence caffeine. [ 14 C]-showdomycin from cells deoxycytidine guanosine. These results suggest is taken up cells.
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