Glutamate Stimulates Oligodendrocyte Progenitor Migration Mediated via an αv Integrin/Myelin Proteolipid Protein Complex

摘要: In the mammalian CNS, oligodendrocyte precursor cells (OPCs) express most neurotransmitter receptors, but their function remains unclear. The current studies suggest a physiological role for glutamate (AMPA and/or kainate) receptors in OPC migration. AMPA stimulated αv integrin-mediated migration by increasing both rate of cell movement and frequency Ca2+ transients. A protein complex containing myelin proteolipid (PLP) integrin modulated AMPA-stimulated migration, stimulation resulted increased association receptor subunits themselves with integrin/PLP complex. Thus, after stimulation, an integrin/PLP/neurotransmitter forms that reduces binding to extracellular matrix enhances To assess extent which PLP was involved OPCs from myelin-deficient (MD) rat, has gene mutation, were analyzed. MD rat had normal basal rate, did not stimulate these cells, suggesting PLP/αv important AMPA-mediated induction. AMPA-induced modulation abolished pertussis toxin, although baseline normal. G-protein-dependent signaling is crucial Other pathways this also determined. These highlight novel determinants could play pivotal regulating