Cytotoxic Effect of 7β-Hydroxycholesterol on Human NCI-H460 Lung Cancer Cells
作者： Kyoung Ah Kang , Sungwook Chae , Kyoung Hwa Lee , Moon Taek Park , Su Jae Lee
DOI: 10.1248/BPB.28.1377
关键词: Fluorescence microscope 、 Cancer research 、 Flow cytometry 、 Lung cancer 、 Caspase 、 Apoptotic body 、 Biology 、 Cytotoxic T cell 、 Apoptosis 、 Cell biology 、 Reactive oxygen species
摘要: The cytotoxic activity of 7β-hydroxycholesterol (7β-OHC) was evaluated on human NCI-H460 lung cancer cells. 7β-OHC decreased clonogenic survival in a dose dependent pattern. induced apoptosis NCI-H460, with the characteristic features like increase sub-G1 hypodiploid (apoptotic) cells, and apoptotic body formation, as evidenced by flow cytometry fluorescence microscope, respectively. Apoptosis also associated loss mitochondrial transmembrane potential, activation caspases 9 3. resulted generation reactive oxygen species (ROS) during apoptosis. On whole, results indicated that inhibited proliferation cells through via caspase activation.
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